rat beta ngf duoset elisa kit  (R&D Systems)

Journal: Journal of Advanced Research

Article Title: Targeting NGF but not VEGFR1 or BDNF signaling reduces endometriosis-associated pain in mice

doi: 10.1016/j.jare.2024.08.017

Figure Lengend Snippet: Levels of VEGFR1 ligands are increased in the peritoneal fluid of endometriosis patients and in mouse lesions. (A) experimental procedures for peritoneal fluid collection from endometriosis patients. (B) The levels of VEGFA, VEGFB, PlGF, and sVEGFR1 in peritoneal fluid samples determined by Ella®. Data are presented in box and whisker charts representing the levels of each mediator in pg/mL. (C) Calculated VEGFR1 occupancy as determined by previously published dissociation constant (K d ) and half maximal effective concentrations (EC 50 ) , , . Note that in published data, occupancy is approximately proportional to signalling . (D) Experimental procedures and timepoints for tissue collection in our mouse model. (E) 56 days after endometriosis induction, mouse lesions were collected for determination of VEGFA levels by ELISA. Results are presented as mean ± SEM of VEGFA levels, n = 10 (sham – uterine horn tissue), and n = 9 (endo) mice per group (*P<0.05 vs. sham by two-tailed Student’s t -test). (F) Profile of VEGFR1 expression in DRG nociceptors determined by immunohistochemistry 56 days after endometriosis induction.

Article Snippet: The concentration of NGF-β from patients’ peritoneal fluid and mouse endometriosis lesions was determined using a commercially available rat beta-NGF DuoSet ELISA kit (Cat# DY556, R&D systems, Minneapolis, MN, USA).

Techniques: Whisker Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Expressing, Immunohistochemistry

Journal: Cells

Article Title: Peripheral Nerve Regeneration–Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion

doi: 10.3390/cells11182887

Figure Lengend Snippet: ELISA with supernatants from neuronally differentiated ADSCs. Expression of NGF is especially upregulated after differentiation with Protocol 3. The obtained values were not normalized to the total cell number.

Article Snippet: The rat NGF Duo Set ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the GDNF (Rat) ELISA Kit (Abnova, Taipei, Taiwan) were used, according to the manufacturers’ instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: Cells

Article Title: Peripheral Nerve Regeneration–Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion

doi: 10.3390/cells11182887

Figure Lengend Snippet: ELISA: expression of NGF after differentiation with Protocols 1, 2, and 3. The obtained values were not normalized to the total cell number.

Article Snippet: The rat NGF Duo Set ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the GDNF (Rat) ELISA Kit (Abnova, Taipei, Taiwan) were used, according to the manufacturers’ instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 3 | NGF and BDNF release from NH2 + + Heparin. (A) NH2 + + Heparin + Immobilised NGF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). NGF at 1 ng/ml in solution in PBS was used as a control (triangles). (B) BDNF release from NH2 + + Heparin + Immobilised BDNF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). BDNF at 1 ng/ml in solution was used as a control (triangles).

Article Snippet: Samples were taken at 1 h, 24 h, 48 h, 168 h (7 days), 240 h (10 days), 336 h (14 days), 408 h (17 days), and 504 h (21 days) and stored at −20°C for later quantification, following the manufacturer’s instructions (NGF and BDNF kit, Cat. No. DY556, DY248, DY008, R&D systems, United States).

Techniques: Control

Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 4 | Average neurite length and Schwann cell migration of DRG when cultured on PCL scaffolds, PCL Air scaffolds, PCL + NH2 + scaffolds, PCL + NH2 + + Heparin scaffolds, and PCL + NH2 + + Heparin + Immobilised neurotrophin for 7 days. (A) Average neurite length and (B) Schwann cell migration for when DRG were cultured with NGF. NGF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. NGF was added in solution at 1 µg/ml with control surfaces. (C) Average neurite length and (D) Schwann cell migration for when DRG were cultured with BDNF. BDNF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. BDNF was added in solution at 1 µg/ml with control surfaces. (E) Average neurite length and (F) Schwann cell migration for when DRG were cultured with NGF plus BDNF. NGF plus BDNF were immobilised on the bioactive surfaces at 1 and 100 ng/ml. NGF plus BDNF were added in solution at 1 µg/ml with control surfaces. One-way ANOVA statistical analysis was performed with Tukey procedure of multiple comparisons *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Samples were taken at 1 h, 24 h, 48 h, 168 h (7 days), 240 h (10 days), 336 h (14 days), 408 h (17 days), and 504 h (21 days) and stored at −20°C for later quantification, following the manufacturer’s instructions (NGF and BDNF kit, Cat. No. DY556, DY248, DY008, R&D systems, United States).

Techniques: Migration, Cell Culture, Control

Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 5 | DRG seeded on control PCL bioactive scaffolds: PCL scaffold, PCL air treated scaffold (PCL Air scaffold), PCL + NH2 + scaffold, PCL + NH2 + + Heparin scaffold, PCL + NH2 + + Heparin + Immobilised NGF at 1 ng/ml scaffold, PCL + NH2 + + Heparin + Immobilised BDNF at 1 ng/ml scaffold, and PCL air treated scaffold (PCL Air scaffold) with NGF plus BDNF at 1 µg/ml in culture medium. B-III tubulin protein in neurites stained in green. S100β of Schwann cells stained in red. Merged channels are yellow, representing the co-localisation of growing neurites and Schwann cells. Scale bar 500 µm.

Article Snippet: Samples were taken at 1 h, 24 h, 48 h, 168 h (7 days), 240 h (10 days), 336 h (14 days), 408 h (17 days), and 504 h (21 days) and stored at −20°C for later quantification, following the manufacturer’s instructions (NGF and BDNF kit, Cat. No. DY556, DY248, DY008, R&D systems, United States).

Techniques: Control, Staining